Research

analysis, biochemistry, biologist, biology, biotechnology, chemistry, clinic, clinical, doctor, equipment, experiment, parties, hospital, laboratory devices, laboratory, lenses, medical, the medicine, microbiology, microscope, the objectives of the, optical, people, copying, study, the science, scientific, technique, test tubes, biology, biology, chemistry, doctor, doctor, doctor, hospital, hospital, laboratory, laboratory, medical, microscope, microscope, microscope, microscope, microscope

Research Themes

Neuroscience

Enabling technologies for chemical neuroscience

Structure

Biomolecular structural characterization and structurally selective quantitation

Microfluidics

Sampling, analysis, and automation at nL – pL and μm – nm scales

Measurements

Measurement science, signal processing, analytical chemistry, and separation science

Instrumentation

New tools for optical detection, separations, biosensing, and microscopy

Fabrication

New approaches in 3D printing and microfabrication

Publications

Selected Publications

These publications are selected to illustrate ongoing research directions:

Online integration of capillary electrophoresis and dual detector Taylor dispersion analysis via a 3D printed instrument

F.S. Atsar, H.D. Bourger, and C.A. Baker

Analyst, 2025,150, 620-629 (DOI: 10.1039/d4an01208a)
We developed a 3D printed instrument for CE-TDA and demonstrated its utility toward standard-free peak identification in enzymatic digestion reactions and for the characterization of thermal denaturation dynamics of heat-resistant proteins. STL files and Instrument designs are freely available for download via the NIH 3D Print Exchange.

3D Printed Instrument for Taylor Dispersion Analysis with Two-Point Laser-Induced Fluorescence Detection

M.R. Moser, C.M. Smith, G.G. Gutierrez and C.A. Baker

Anal. Chem. 2022, 94, 16, 6089–6096 (DOI:10.1021/acs.analchem.1c04566)
This was the predecessor to our more recent CE-TDA instrument. This work established an innovative optical and fluidic design fabricated by 3D printing, and demonstrated the utility of TDA-LIF for high sensitivity structural characterization of proteins.

Taylor dispersion analysis in fused silica capillaries: a tutorial review

M.R. Moser, and C.A. Baker

Anal. Methods, 2021,13, 2357-2373 (DOI:10.1039/D1AY00588J)
This is a concise guide to the fundamental principles of molecular sizing by Taylor disperpersion analysis (TDA) and the advantages of performing this analysis at microfluidic scales. Practical considerations and applications from literature are also discussed

A microfluidic bubble perfusion device for brain slice culture

A. Saleheen, D. Acharyya, R.A. Prosser, and C.A. Baker

Anal. Methods, 2021,13, 1364-1373 (DOI:10.1039/D0AY02291H)
We developed a microfluidic perfusion system for delivering gaseous oxygen and liquid media to ex vivo brain slice cultures. The system mimiced the roller tube culture method while integrating with fluorescence microscopy for physiological studies of induced Ca2+ flux

More Information:

Follow these links for a complete list of our publications and the grants and agencies that support our work